Frequently asked questions

58Is OD600 truly absorption?59How can OD600 be converted to cell count?

Knowledge Library

1Why do absorbance and fluorescence give different concentrations for the same DNA sample?2Why can the wrong blank change a nucleic acid concentration result?3Choosing the right conversion coefficient for A280 protein quantification4A high standard-curve R² does not guarantee accurate concentrations

How can OD600 be converted to cell count?

Establish an empirical curve using CFU or another independent count for the specific organism, culture conditions and instrument. A fixed generic conversion factor is not a universal true value.

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