Frequently asked questions

48What is the basic principle of the BCA assay?49What commonly interferes with the BCA assay?50What is the basic principle of the Bradford assay?51Why does the Bradford assay respond differently to different proteins?52What is the basic principle of the Lowry assay?53Why do colorimetric protein assays require a standard curve?54What might cause a flattened spectral peak?55Why can absorbance be negative?56What should I check for a jagged spectrum or large differences between repeats?57Why can different instruments give different results for the same sample?

What commonly interferes with the BCA assay?

Reducing agents, chelators and strong acids or bases may interfere with the copper reaction. Check compatibility tables and use a matrix-matched standard curve.

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Main measurement needs