Frequently asked questions

38Can pH affect A260/A280?39What is A260/A230 used for?40Why are purity ratios often unstable at low concentration?41Can UV absorbance distinguish dsDNA, ssDNA and RNA?42Why does RNA contamination lead to an overestimate of DNA concentration?43How do absorbance and fluorescence methods differ?44Can purity ratios indicate nucleic acid integrity?45Why can proteins be quantified directly using A280?46Can all proteins use the same A280 conversion factor?47What are the limitations of direct A280 quantification?

Why does RNA contamination lead to an overestimate of DNA concentration?

RNA also contributes to A260. A calculation using the dsDNA factor includes this additional absorption.

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