31Which metrics should be considered when evaluating a high-performance spectrometer?32Why are nucleic acids commonly quantified at 260 nm?33Can dsDNA, RNA and ssDNA use the same conversion factor?34Why are sequence-specific extinction coefficients preferable for oligonucleotides?35What does A260/A280 represent?36What are common A260/A280 reference values for DNA and RNA?37Does a normal A260/A280 mean a sample is definitely pure?38Can pH affect A260/A280?39What is A260/A230 used for?40Why are purity ratios often unstable at low concentration?

Can pH affect A260/A280?

Yes. Studies show that pH and ionic strength change nucleic acid spectral ratios. Keep buffer conditions as consistent as possible when comparing samples.