Frequently asked questions

28Why does baseline stability matter?29What is photometric linearity?30Are calibration and verification the same?31Which metrics should be considered when evaluating a high-performance spectrometer?32Why are nucleic acids commonly quantified at 260 nm?33Can dsDNA, RNA and ssDNA use the same conversion factor?34Why are sequence-specific extinction coefficients preferable for oligonucleotides?35What does A260/A280 represent?36What are common A260/A280 reference values for DNA and RNA?37Does a normal A260/A280 mean a sample is definitely pure?

Why are sequence-specific extinction coefficients preferable for oligonucleotides?

Base composition and neighboring-base effects change absorption. A sequence-specific extinction coefficient is generally more suitable than a generic empirical factor.

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