31Which metrics should be considered when evaluating a high-performance spectrometer?32Why are nucleic acids commonly quantified at 260 nm?33Can dsDNA, RNA and ssDNA use the same conversion factor?34Why are sequence-specific extinction coefficients preferable for oligonucleotides?35What does A260/A280 represent?36What are common A260/A280 reference values for DNA and RNA?37Does a normal A260/A280 mean a sample is definitely pure?38Can pH affect A260/A280?39What is A260/A230 used for?40Why are purity ratios often unstable at low concentration?

Can dsDNA, RNA and ssDNA use the same conversion factor?

No. Common empirical factors differ, so the correct sample type and method settings must be selected for the application.