11Why can a liquid column remain between the measurement surfaces?12Why do microvolume measurements often use a short path length?13What does normalization to a 1 cm path length mean?14Why does path-length error directly affect concentration?15How should a stated path-length control specification be interpreted?16Why might different concentrations need different path lengths?17How can consistency between multiple short path lengths be verified?18What is a broken liquid column?19Which samples are more prone to liquid-column problems?20Why do bubbles affect results?

Which samples are more prone to liquid-column problems?

Samples with low surface tension, detergents, unusual viscosity or difficult pipetting are at greater risk. Observe the column and repeat measurements.