1dsDNA absorbance quantification 2RNA absorbance quantification 3Clues to nucleic acid purity 4Purified protein quantification at A280 5BCA total protein quantification 6Bradford total protein quantification 7UV-Vis full-spectrum scanning 8dsDNA fluorescence quantification 9RNA fluorescence quantification 10Microbial growth curves

UV-Vis full-spectrum scanning

Where are the absorption features of a purified compound, pigment or reaction product?

Method: Record peak position, bandwidth, baseline and absorbance using a matched Blank.

Considerations: Solvent cutoff wavelength, pH, concentration, path length and container can all change the result.