1dsDNA absorbance quantification 2RNA absorbance quantification 3Clues to nucleic acid purity 4Purified protein quantification at A280 5BCA total protein quantification 6Bradford total protein quantification 7UV-Vis full-spectrum scanning 8dsDNA fluorescence quantification 9RNA fluorescence quantification 10Microbial growth curves

dsDNA absorbance quantification

How much purified PCR product, plasmid or genomic DNA is present?

Method: Estimate concentration from A260 and the conversion factor for the correct sample type.

Considerations: Protein, phenol, guanidinium salts, residual RNA and Blank mismatch may all elevate results.